Expression and effect of THEM4/Akt on extracellular matrix deposit in kidney of diabetic mice
10.13315/j.cnki.cjcep.2018.02.011
- VernacularTitle:THEM4/Akt在糖尿病小鼠肾脏细胞外基质沉积中的作用
- Author:
Ning CHEN
1
;
Jun HAO
;
Xiao-Ying AN
;
Gui-Yun ZHU
;
Li-Fei KANG
;
Xiao-Xia LI
;
Yong-Hui YANG
Author Information
1. 河北省胸科医院病理科
- Keywords:
diabetes mellitus;
renal tubular cells;
extracellular matrix deposit;
thioesterase superfamily member 4;
PI3 K/Akt
- From:
Chinese Journal of Clinical and Experimental Pathology
2018;34(2):173-177,182
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the relations of THEM4/Akt expression and extracellular matrix deposit in kidney of diabetic mice. Methods Diabetic mice models were successfully established by intraperitoneally injected STZ. Both normal control mice and diabetic mice were raised for 8 week until they were sacrificed. Western blot, immunohistochemistry and realtime PCR were used to detect the expression of THEM4, phospho-Akt (Ser 473), TGF-β1, a-SMA, Col Ш, FN protein and THEM4 mRNA in the kidneys of normal mice and diabetic mice. Results Compared to normal control mice, THEM4 expression decreased by 37.7％ followed by 3.66, 1.29 2.33, 1.99 and 2.82 times increased of phospho-Akt (Ser473), TGF-β1, a-SMA, Col Ш and FN in kidney of diabetes mellitus. Extracellular matrix accumulation was found in renal interstitial region of diabetic mice. Conclusion The decreased THEM4 might cause extracellular matrix deposit in kidney of diabetic mice by upregulating the phosphorylation of Akt and TGF-β1, α-SMA expression in diabetic mice.
