Consistency and clinicopathologic significance of BRAFV600E protein expression and genic mutation in papillary thyroid carcinoma
10.13315/j.cnki.cjcep.2018.01.010
- VernacularTitle:甲状腺乳头状癌中BRAFV600E蛋白表达与基因突变的一致性及其临床意义
- Author:
Yan-Ting L(U)
1
;
Hai-Yong ZHANG
;
Fei-Xing ZHAO
;
Xu-Lian LU
;
Xiu-Fang LI
;
Meng-Jun HU
Author Information
1. 浙江省诸暨市人民医院 病理科 311800
- Keywords:
papillary neoplasm;
thyroid carcinoma;
BRAFV600E;
consistency;
clinicopathologic correlation
- From:
Chinese Journal of Clinical and Experimental Pathology
2018;34(1):42-45
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the consistency and clinicopathologic correlation of BRAFV600E protein expression and gene mutation in papillary thyroid carcinoma. Methods BRAFV600E protein expression and genn mutation was detected respectively by immunohistochemistry of SP and real time-PCR, then the consistency between the both methods was analyzed by Kappa-test, the correlation between BRAFV600E and clinicopatho-logic parameters was analyzed by Chi-square test in papillary thyroid carcinoma. Results The gene mutation and protein expression rates of BRAFV600E were 89.3％ and 88.3％, respec-tively, the differences were not significant, the concordance rate of the both methods was 97.0％, Kappa value was 0.847, the consistence was higher, meanwhile the mutation rates between age ＜45 and ≥45 were respectively 96.8％ and 85.9％, there were significant differences, the positive rates of the both detec-tion methods were higher in thyroid capsule invaded group than non-invaded group, the differences were significant. Conclusion The both methods have higher consistency, the immunohisto-chemistry can be used as an initial screening tool for detecting gene mutation, the gene mutation of BRAFV600E is significantly associated with age and capsule invasion, the relationship is not found between BRAFV600E mutation and the other clinicopatholog-ic parameters.
