Expression of CHMP4A and TSPYL-2 in early esophageal cancer and their significance
10.13315/j.cnki.cjcep.2017.12.007
- VernacularTitle:食管癌早期基因CHMP4A及TSPYL-2在食管癌变过程中的表达及其意义
- Author:
Rong YANG
1
;
Li-Na SHANG
;
Gui MA
;
Li-Juan SONG
;
Chun-Xia YANG
;
Wei LIU
;
Xin HE
;
Ya-Qiong KANG
;
Yan LUO
;
Hai-Ke XIONG
Author Information
1. 甘肃省肿瘤医院病理诊断中心
- Keywords:
esophageal neoplasms;
squamous cell carcinoma;
field cancerization;
suppression subtractive hybridization;
CHMP4A;
TSPYL-2
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(12):1324-1327
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the role of CHMP4A and TSPYL-2 in early pathogenesis of esophageal cancer.Methods Through comparison of the four subtractive libraries,early esophageal squamous cell carcinoma genes CHMP4A and TSPYL-2 were chosen for further study.Through RT-PCR and immunohistochemistry methods,CHMP4A and TSPYL-2's expression was detected in esophageal squamous cell carcinoma tissue,cancerous tissue and normal esophageal mucosa.Results CHMP4A and TSPYL-2 expression between esophageal squamous cell carcinoma and normal esophageal epithelium tissue had significant differences (P ＜ 0.05),and the CHMP4A gene expression in esophageal mucosa,field cancerization areas,esophageal squamous cell carcinoma tissue increased,while TSPYL-2 gene expression in esophageal mucosa,field cancerization areas,esophageal squamous cell carcinoma tissue decreased,which were consistent with the protein expression of CHMP4A and TSPYL-2.Conclusion CHMP4A and TSPYL-2 genes are differentially expressed in esophageal squamous cell carcinoma,which can be used as alternative genetic markers for further research.
