Effect of silencing CD98hc expression on MDA-MB-231 breast cancer cells proliferation and invasion

VernacularTitle:沉默CD98hc表达对乳腺癌细胞MDA-MB-231增殖、侵袭的影响
Author: Lang MOU ¹ ; Zheng-Sheng WU ; Qiang WU
Author Information

1. 安徽医科大学病理学教研室
Keywords: breast neoplasm; CD98hc; cell proliferation; cell invasion
From: Chinese Journal of Clinical and Experimental Pathology 2017;33(11):1199-1202
CountryChina
Language:Chinese
Abstract: Purpose To investigate the effect of silencing of CD98hc on proliferation,migration and invasion of MDA-MB-231 breast cancer cells.Methods RNA interfere technology was used to silence CD98hc in MDA-MB-231 cells.MDA-MB-231 cells (CD98hc-shRNA) with stable low expression of CD98hc and Vector control was obtained.Western blot and qRT-PCR were used to verify the down-regulation of CD98hc.MTT assay was used to test the cell proliferation.Transwell migration and invasion experiment were used to assay cell migration and invasion.Results The expression levels of CD98hc was down-regulated by CD98hc-shRNA in the MDA-MB-231 breast cancer cells.Compared to that of blank cells (0.706 ± 0.013),vector control (0.724 ± 0.018),the cell proliferation potency of CD98hc-shRNA cells (0.580-0.035) was significantly inhibited (P ＜ 0.05),and the cell migration and invasion potency also were inhibited (P ＜ 0.05),there is on significant different between blank cells and vector control (P ＜ 0.05).Conclusion CD98hc might be involved in the regulation of breast cancer cell biological behaviors.