MiR-21 promotes the EMT and invasion of prostate cancer cell by inducing FOXO1
10.3969/j.issn.1005-6483.2018.03.019
- VernacularTitle:miR-21诱导FOXO1调控前列腺癌细胞EMT及侵袭转移
- Author:
Ying-Hu CHEN
1
,
2
;
Zhenting WANG
;
Chaohui ZHONG
Author Information
1. 410399 长沙,中南大学湘雅二医院泌尿外科
2. 解放军第四二五医院外一科
- Keywords:
prostate cell;
FOXO1;
miRNA-2;
EMT
- From:
Journal of Clinical Surgery
2018;26(3):223-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of EMT and invasion promoted by miR-21 in prostate cancer cells. Methods The sequence of miR-21 mimic/inhibitor was firstly designed and synthesized. Then miR-21 mimic/inhibitor and its control were transfected into prostate cancer cells C4-2 and DU145, respectively. And cells were collected for mRNA isolation and RT-qPCR analysis for miR-21 and FOXOl. FOXOl, E-cadherin and N-cadherin were detected by Western blot, and the invasion of prostate cancer cells were detected by transwell assays. Results The expression of miR-21 increased in both C4-2 and DU145 after transfection, and the expression of FOXOl mRNA increased at the same time (P＜0.01).The expression of miR-21 and FOXOl mRNA in C4-2 and DU145 was decreased by miR-21 inhibitor(P＜ 0.05). The protein expression of FOXOl and N-cadherin in C4-2 and DU145 increased after the treatment of miR-21, while that of E-cadherin decreased. The protein expression of FOXOl and N-cad-herin in C4-2 and DU145 decreased after the treatment of miR-21 inhibitor, while that of E-cadherin increased. The invasive level in C4-2 and DU145 increased after the treatment of miR-21, while that decreased after the treatment of miR-21 inhibitor. Conclusion MiR-21 promotes EMT and invasion by inducing FOXOl in prostate cancer cells.
