Effects of nimodipine combined with 7.5％ hypertonic saline on sevoflurane-induced apoptosis of hippocampal neuron in aged rats
- VernacularTitle:尼莫地平复合7.5％高渗盐水对七氟醚诱导老龄大鼠海马神经元凋亡的影响
- Author:
Qi ZHANG
1
;
Yanan LI
;
Peng GUO
;
Xi XIN
;
Shuping HUO
;
Qiujun WANG
Author Information
1. 河北医科大学第三医院麻醉科
- Keywords:
Nimodipine;
Hypertonic saline;
Aged rats;
Neuronal apoptosis
- From:
The Journal of Clinical Anesthesiology
2017;33(12):1215-1219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of nimodipine combined with 7.5％ hypertonic saline (HS) on sevoflurane-induced apoptosis of hippocampal neuron in aged rats.Methods Ninetysix healthy male Wistar rats aged 18 months and weighing 450-500 g were randomly assigned into 4 groups (n=24 each):group C,group N,group HS and group NHS.Group N received intraperitoneal injection of 1 mg/kg nimodipine and intravenous injection of normal saline,group HS received intravenous injection of 4 ml/kg 7.5％ HS and intraperitoneal injection of normal saline,group NHS received intraperitoneal nimodipine and intravenous HS mentioned above and group C received normal saline.Thirty minutes later,4 groups inhaled 3％ sevoflurane for 2 hours.Morris water maze test was performed 1 day before anesthesia and 1,3 and 7 days after anesthesia.Morris water maze test was carried out 1 day before anesthesia and 1 and 7 days after anesthesia,8 rats were sacrificed and brains were removed.Hippocampal tissues were obtained for detection of apoptosis in hippocampal neurons,intracellular [Ca2+]i by flow cytometry and the measurement of Bcl-2 and Bax mRNA expression by RT-PCR.Results Compared with group C,the escape latency,apoptotic rate,[Ca2+]i,Bax mRNA expression and Bax/Bcl-2 ratio were significantly decreased,the frequency of crossing the original platform and Bcl-2 mRNA expression increased in groups N,HS and NHS after anesthesia (P＜0.05).Compared with group NHS,the escape latency,apoptotic rate,[Ca2+]i,Bax mRNA expression and Bax/Bcl-2 ratio were significantly increased,the frequency of crossing the original platform and Bcl-2 mRNA expression were decreased in groups N and HS after anesthesia (P ＜ 0.05).Conclusion Nimodipine combined with 7.5％ HS could reduce apoptosis rate of sevoflurane-induced hippocampal neuron by inhibiting calcium overload in aged rats,and it exerts better protective effects than single drug administration.
