Identification for rare B(A) blood group

VernacularTitle:罕见B(A)血型的鉴定
Author: Li XIONG ¹ ; Xiaoying ZHOU ; Hong WU ; Qi TANG ; Guoliang LI
Author Information

1. 江西省血液中心输血研究室
Keywords: B(A)subgroup; blood group serology; PCR-SSP genotyping; direct sequencing; transfution
From: Chinese Journal of Clinical Laboratory Science 2018;36(6):426-428
CountryChina
Language:Chinese
Abstract: Objective To investigate the serological and molecular identification of 2 rare B( A) blood groups. Methods The ABO blood groups of 2 samples from blood donors were detected by routine serological method. The genotype features was identified by PCR-sequence specific primer (PCR-SSP) and direct sequence analysis. Results The serological results for the 2 blood donors showed the characteristics of B(A) phenotype. The sample 1 was genotyped as BO2 subtype by PCR-SSP and direct sequencing showed B alleles in exon 7, presented nt640 A＞G mutation which was confirmed to be B(A)04/O02 genotype.The sample 2 was genotyped as BO1 sub-type by PCR-SSP and direct sequencing showed B alleles presented nt700 C＞G mutation in exon 7 which was confirmed to be B(A)02/O01 genotype. Conclusion The phenotype of the two samples should be B ( A ) and the genotypes should be rare B(A)04/O02 and B(A)02/O01.