Establishment of internal quality control system for microbiological identification by MALDI-TOF MS
10.13602/j.cnki.jcls.2018.04.16
- VernacularTitle:MALDI-TOF MS微生物鉴定的室内质量控制体系建立
- Author:
Xiuyuan LI
1
;
Yanan LI
;
Xinxin LU
Author Information
1. 首都医科大学附属北京同仁医院检验科
- Keywords:
matrix-assisted laser desorption ionization-time of flight mass spectrometry;
microbiological identification;
internal quality control
- From:
Chinese Journal of Clinical Laboratory Science
2018;36(4):297-303
- CountryChina
- Language:Chinese
-
Abstract:
The matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is one of the first choice techniques for clinical microbiological identification owing to its fast,accuracy,low cost and easy to be operated.The accuracy of identification of MALDI-TOF MS relies on good instrument status,appropriate pre-trement,standardized operation and the rules of interpretation.Any errors in this process will directly influence the accuracy of identification.Therefore,strict internal quality control for the microbiological identification by MALDI-TOF MS is very important.Based on six years of research and clinical application experience,our laboratory establishes a relatively complete internal quality control system for the microbiological identification by MALDI-TOF MS,including all aspects of hardware,software and manual operation,the parameter settings,maintenance and calibration of the instrument,the preparation of strains and reagents,spectra acquisition and the interpretation and analysis of results,Which can make abnormal results be quickly traced and timely handled.
