Effects of three kinds of liquid media on the biofilm formation of Staphylococcus epidermidis
10.13602/j.cnki.jcls.2018.04.02
- VernacularTitle:3种液体培养基对表皮葡萄球菌生物膜形成的影响
- Author:
Juan ZHU
1
;
Pengfei SHE
;
Ruichen TAN
;
Yanle WANG
;
Yong WU
Author Information
1. 中南大学湘雅三医院检验科
- Keywords:
Staphylococcus epidermidis;
LB medium;
TSB medium;
M-H medium;
ica gene;
biofilm
- From:
Chinese Journal of Clinical Laboratory Science
2018;36(4):245-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 3 kinds of broth media,including tryptose soya broth(TSB),LB and M-H,on the biofilm formation of Staphylococcus epidermidis (S.epidermidis).Methods The effects of TSB,LB and M-H broth media on the biofilm formation of S.epidermidis were investigated by the construction of bacterial biofilm in 96-well and 6-well microplates and the crystal violet straining for the semi-quantitative analysis and microscopic observation of the bacterial biofilm.The effects of TSB,LB and M-H broth media on the expression of adhesion-related genes in S.epidermidis were determined by the extraction of bacterial total RNA,reverse transcription and real-time PCR.Results Compared with LB (0.149 ± 0.047) and M-H (0.323 ± 0.003) media,TSB medium (2.954 ± 0.287) could significantly promote the biofilm formation of S.epidermidis (TSB vs LB,t =16.706,P ＜ 0.01;TSB vs M-H,t =15.877,P ＜ 0.01).Compared with LB medium,TSB medium could significantly enhance the expression of icaA gene (t =9.667,P＜0.01) but inhibit icaR gene (t =13.283,P＜0.01).Conclusion Compared with LB and M-H broth media,TSB medium may significantly improve the biofilm formation and the expression of adhesion-related gene icaA of S.epidermidis.
