A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos
10.3969/j.issn.1003-4706.2018.06.021
- VernacularTitle:印记基因H19在不同质量精子和胚胎中的甲基化状态
- Author:
Li－feng XIANG
1
;
Qing－qing WANG
;
Ming－ying LI
;
Peng ZENG
;
Yong－gang LI
Author Information
1. 云南省第一人民医院生殖医学科
- Keywords:
Imprinting gene H19;
Sperm;
Embryo;
Methylation;
ART
- From:
Journal of Kunming Medical University
2018;39(6):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective By comparing the methylation status of imprinted gene H19 in three different quality spermatozoa and the methylation status of the embryos after the combination of spermatozoa, the sperm methylation status in the ART technique was preliminarily explored with the postnatal embryo methylation.Methods In this study, a total of 91 spermatozoa and 91 low－quality embryos were collected from IVF－ET in the First People's Hospital of Yunnan Province. Among them, sperm samples were divided into three groups according to the sperm concentration parameter in the WHO fourth edition,then the relationship between different quality spermatozoa and postnatal embryo methylation were analyzed.Results The abnormality rate of H19 methylation status in group B was significantly higher than that of the sperm group and the embryo group.Conclusions The abnormalities of H19 methylation mainly concentrated in the abnormal parameters of spermatozoa,suggesting that the abnormal state was related to the decrease of sperm quality;Through IVF, ICSI fertilized embryos have the same proportion of the corresponding abnormal state with no significant difference; Abnormal sperm embryos did not show abnormality, which may be related to the self－repair function of the embryo itself.
