The Anti-antioxidant Role of Ginsenoside Rg1 Regulating Nrf2 on Focal Cerebral Ischemia Reperfusion SD Rats
10.3969/j.issn.1003-4706.2018.06.008
- VernacularTitle:人参皂苷Rg1调控Nrf2在SD大鼠脑缺血再灌注损伤后的抗氧化作用
- Author:
Li－gong BIAN
1
;
Lian－mei ZHONG
;
Qing－long AI
;
Xin－yue CHEN
;
Wen－kai XU
;
Run－qi YAN
;
Jin QIU
;
Di LU
Author Information
1. 昆明医科大学人体解剖学与组织胚胎学系
- Keywords:
Ginsenoside Rg1;
Ischemia/Reperfusion injury;
Heme oxygenase－1;
Superoxide orgotein dis－mutase;
Malondialdehyde
- From:
Journal of Kunming Medical University
2018;39(6):35-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study antioxidant role and mechanism of Rg1 in rats with cerebral ischemia reperfusion injury (IRI).Methods One hundred and twenty healthy male rats were randomly divided into six groups: control group, sham operation group, model group, different concentration (30,60,90 mg/kg) of Rg1 treatment group.MCAO SD rats model was established by suture－occluded method;the Rg1 treatment groups were given Rg1 i.p. in advance, after 24 hours of reperfusion, neurobehavioral scores of all groups were examined by Longa’s standard;The expression of Nrf2 and HO－1 were analyzed by western blot;The content of SOD and MDA was detected by kit.Results The score of model group rats are significantly higher than control group,compared with the model group, the score of different concentration of Rg1 treatment group was decreased (P＜0.05) . The Nrf2 and HO－1 expression in model group was mildly higher than the control or sham group (P＜0.05) . Both of them in every Rg1 treatment group was higher than model group. Compared with control or sham group, SOD content was observably depressed but MDA content was dramatically increased in model group,interestingly,SOD content was enhanced, MDA content was attenuated in different concentration of Rg1 treatment group (P＜0.05). Conclusion Rg1 increases Nrf2 and HO－1 protein expression and SOD content, reduces MDA content,improves neurofunctional performance of rats after MCAO,and alleviates cerebral cerebral IRI.
