Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method
10.3969/j.issn.1003-4706.2018.05.005
- VernacularTitle:UPLC-MS/MS法研究滇乌碱在大鼠体内组织的分布
- Author:
Fa-Huan LU
1
;
Ji-Yin LI
;
Shu-Hua LI
;
Wen-Song ZHAO
;
Rui WANG
;
Run-Fang XIE
;
Kai-Run YANG
Author Information
1. 腾冲市公安局
- Keywords:
Aconitum vilmorinianum Kom;
Yunaconitine;
UPLC-MS/MS;
Rat;
Tissue distribution
- From:
Journal of Kunming Medical University
2018;39(5):16-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an acute yunaconitine poisoning rat model with a single oral administration and to determine the contents of yunaconitine in rat tissues by UPLC-MS/MS method, then investigate the distribution of yunaconitine in rats. Method The rats were randomly divided into three groups and were intragastrically administered a single dose of 2.2mg/kg,1.1mg/kg,0.7mg/kg yunaconitine, respectively.. The rats were killed 2h later, the stomach tissue, intestine tissue, liver tissue, pancreas tissue, kidney tissue, lung tissue, spleen tissue, heart tissue, bladder tissue, testis tissue, brain tissue and heart blood samples were collected. The contents of yunaconitine in the biological materials were determined by UPLC-MS/MS method after the biological samples extracted by liquid-liquid extraction. Result A rat model of the yunaconitine poisoning was made with a single dose of 1.1mg/kg, the concentrations of yunaconitine displayed in the organs with the following order:stomach, small intestine, liver, pancreas, kidney, lung, spleen, heart, bladder, testis, heart blood and brain. Conclusion Yunaconitine was widely distributed in rats, especially the levels in the stomach, small intestine and liver were the highest. The conclusion provides a basis for the selection of test materials for the poisoning of Aconitum vilmorinianum Kom.
