The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo

VernacularTitle:单抗KMP1的体内抑制膀胱癌EJ细胞株成瘤的动物实验
Author: Can YANG ¹ ; Yu-Hang GONG ; Hai-Feng WANG ; Hai-Hao LI ; Jing-Yu LIU ; Wei WANG ; Jian-Song WANG ; Yi-Gang ZUO ; Jian CHEN ; Hui ZHAN ; Ming-Xia DING
Author Information

1. 昆明医科大学第二附属医院泌尿外科
Keywords: Bladder carcinoma; EJ cells; Monoclonal antibody
From: Journal of Kunming Medical University 2018;39(5):1-6
CountryChina
Language:Chinese
Abstract: Objective To research the monoclonal antibody KMP1 inhibited bladder cancer EJ cell lines growth and metastasis in vivo by bioluminescence imaging. Methods Immunohistochemistry was used to determine the KMP1 binding to EJ and EJ-GFP cell lines. The xenograft tumor cell growth and distribution were measured by vernier calipers and dynamic in vivo fluorescence imaging. Immunohistochemistry and H&E counterstaining researched the feature of the xenograft tumor. Results Cell growth curves of EJ and EJ-GFP cells were similar. EJ-GFP had a green fluorescence. In EJ-GFP nude mouse tumor model, the addition of KMP1 significantly inhibited tumor growth and extended the average life span of nude mice. Both EJ and EJ-GFP cells can bind to KMP1,and the weight of transplanted tumors in the KMP1 treatment group was significantly lower than that of the mIgG control group (P＜0.001).Conclusion KMP1 has a promising antitumor effect in vivo. It might be valuable for development as a promising targeted agent for bladder cancer.