Serotyping and PFGE Type of Salmonella Isolates in Yunnan Province
10.3969/j.issn.1003-4706.2018.02.007
- VernacularTitle:云南省食源性沙门菌血清分型及脉冲场凝胶电泳的指纹图谱
- Author:
Jiang ZHAO
1
;
Xiang-Dong MIN
;
Tong-Jian CAI
;
Xing-Meng HAN
;
Zu-Shun YANG
;
Zhi-Tao LIU
Author Information
1. 云南省疾病预防控制中心
- Keywords:
Salmonella;
Serotype;
Salmonella typhimurium;
Pulsed field gel electrophoresis;
Molecular typing;
Foodborne pathogen
- From:
Journal of Kunming Medical University
2018;39(2):30-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the serotypes and molecular characteristics of foodborne Salmonella in Yunnan Province using pulsed field gel electrophoresis (PFGE) and to establish PFGE fingerprint database. Methods This study was carried out on the basis of the Serological typing of 322 strains of foodborne Salmonella which was isolated from Yunnan national foodborne disease surveillance from 2013 to 2016. The clustering analysis was conducted on 148 strains of Salmonella DNA restriction enzyme map by using the software of BioNumerics. Fingerprint database was established through the comparison of clustering analysis correlation on bacterial strain. Results The serotype of 322 strains of Salmonella mainly included A, B, C, D, E, F, G and other 7 groups,among which Salmonella typhimurium was the major type, accounting for 11.4% (37/322) . Cluster analysis was applied using BioNumerics software in 148 strains of Salmonella DNA restriction enzyme map. According to the different number and the different positions, electrophoresis strips were divided into 102 different PFGE patterns (Figure 1) , which was categorized into 39 clusters if 90% of the strips was similar. Conclusion Foodborne salmonella molecular classification is complex. Salmonella typhimurium is the major type. PFGE belt type presents diversity.
