An Analysis of Movement Function of SD Rats'Spinal Cord Injury After Joint Therapy Using NT- 3- HUMSCs and SOCS3 Gene Silencing
10.3969/j.issn.1003-4706.2018.02.004
- VernacularTitle:NT-3-HUMSCs联合基因沉默SOCS3治疗SD大鼠脊髓损伤后的运动功能分析
- Author:
Gang BAI
1
;
Hong-Tian ZHANG
;
Jun LAI
;
Lin LUO
;
Pin ZUO
;
Yao-Dong FAN
Author Information
1. 昆明医科大学第三附属医院
- Keywords:
Spinal cord injury;
SOCS3;
siRNA;
NT-3 gene;
Gene transfection
- From:
Journal of Kunming Medical University
2018;39(2):15-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To achieve the purpose of promoting movement function of the injury nerve by using the joint therapy of NT- 3- HUMSCs and SOCS3 gene silencing on SD rats'spinal cord injury. Methods (1) We used adherence method in vitro human umbilical cord-derived mesenchymal cells (HUMSC) during separation, purification and identification. (2) Then constructed NT-3 gene eukaryotic expression vector, which was transfected into its HUMSC, and constructed NT-3- HUMSC cell survival in vitro assay conditions and NT-3 expression. (3) We selected specific targets for SOCS3 screening and for sequence homology analysis. A negative control group was established. siRNA was designed and synthesized in vitro detection. (4) SD rats with spinal cord injury model were divided into two categories: (1) sham group with 10 rats; (2) T12 whole spinal cord injury model with 40 rats. The 40 rats were randomly divided into four groups with 10 rats in each group (saline treatment group,siRNA +NT-3-HUMSCs treatment group,NT-3-HUMSCs treatment group and siRNA treated group) . Motor function of the rats were evaluated respectively in 1, 2 and 3 months after the modeling was established successfully.Results(1) siRNA + NT-3-HUMSCs treatment group's BBB scores was significantly higher than NT-3-HUMSCs, SOCS3-siRNA and physiological saline groups ( P＜0.05) . (2) The grid climbing experiments showed that the neural functional recovery performed better in siRNA+the NT- 3- HUMSCs treatment group compared to the NT - 3 - HUMSCs, SOCS3 - siRNA and physiological saline groups (P＜0.05) . Conclusion The NT- 3- HUMSCs joint SOCS3 gene silencing in the treatment of SD rat spinal cord injury can improve the motor function of SD rat spinal cord injury.
