The Suppressive Effect of Carthamin Yellow on the Proliferation and Migration of Human Breast Cancer Cells and Its Related Molecular Mechanisms
10.3969/j.issn.1003-4706.2018.01.005
- VernacularTitle:红花黄色素对乳腺癌细胞增殖和迁移的抑制作用及其分子机制
- Author:
Xiang-Dong KONG
1
;
Shao-Feng YUAN
;
Liang-Ming PAN
;
Lin-Wen ZENG
;
Ming WU
;
Jian-Liang SHEN
Author Information
1. 上海市金山区亭林医院外科
- Keywords:
Carthamin Yellow;
Breast cancer cell;
Proliferation;
Apoptosis;
Migration and invasion
- From:
Journal of Kunming Medical University
2018;39(1):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Carthamin Yellow (CY) on cell proliferation, apoptosis, migration and invasion ability of breast cancer and its related molecular mechanisms. Methods CCK-8 assay was used to detect cell viability of MDA-MB-231 human breast cancer cells by different concentrations of CY at different time;flow cytometry was used to test the apoptosis rate of MDA-MB-231 cells treated by different concentrations of CY and transwell assay was used to investigate the effect of various concentrations of CY on MDA-MB-231 cell migration and invasion.After the intervention of different concentrations of CY on MDA-MB-231 cells, apoptosis-related protein Cleaved-Caspase-3, survival protein p-Akt and metastasis-related protein MMP2 were detected by western blot. Results (1) CY could inhibit the proliferation of MDA-MB-231 cells in a dose-and-time-dependent manner. (2) CY significantly promoted the apoptosis of breast cancer cells ( <0.01) . (3) CY could decrease the expression of p-Akt and increase the expression of Cleaved-Caspase-3. (4) CY impaired migration and invasion of MDA-MB-231 cells ( <0.01), and can inhibit the expression of MMP2. Conclusion CY could promote the apoptosis of breast cancer cells through activation of apoptosis signaling, and can inhibit breast cancer cell metastasis by suppressing MMP2. And CY may be a potential therapeutic drug for human breast cancer.
