Model of cardiac arrest in rats established by modified transcutaneous electrical stimulation on epicardium
10.3760/cma.j.issn.1671-0282.2018.05.011
- VernacularTitle:改良经皮心外膜电刺激建立大鼠心脏骤停模型
- Author:
Tingting SHU
1
,
2
;
Yuhan ZHANG
;
Licai LIANG
;
Lang GUO
;
Li XU
;
Weijing SHAO
;
Peng SUN
;
Jiyuan HAN
Author Information
1. 430022 武汉,华中科技大学同济医学院附属协和医院急诊科
2. 华中科技大学同济医学院附属梨园医院中西医结合科
- Keywords:
Ventricular fibrillation;
Cardiac arrest;
Modified method;
Electrical epicardium stimulation;
Rat
- From:
Chinese Journal of Emergency Medicine
2018;27(5):513-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the cardiac arrest (CA) model in rats by modified transcutaneous electrical stimulation on epicardium. Methods This study was performed in the Emergency Medicine laboratory in Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. After 10 Sprague-Dawley male rats weighing 330-380 g were anesthetized, two acupuncture needles connected to the anode and cathode of a stimulator were transcutaneously inserted into the epicardium as electrodes. The puncture points were located quantitatively according to the anatomical structure of the rat chest. The electrical stimulation was maintained for 3 minutes to induce ventricular fibrillation(VF). Cardiopulmonary resuscitation (CPR) included chest compressions, intravenous adrenaline and defi brillation operated at 6 min after a period of nonintervention. Results CA was induced after the implement of the effective electrical stimulation in all ten rats in this experiment. The average current intensity to induce VF was (1.80 ± 0.59) mA, the average time to induce CA was (5.07 ± 2.37)s,the average time of the total electrical stimulation was(187.50 ± 12.75)s and the total time of CA was 6 min. At the end of the electrical stimulation, 9 rats presented VF and 1 rat showed pulseless electrical activity. The restoration of spontaneous circulation was achieved in all 10 rats. The average time of CPR was(190.90±68.60) s, the mean numbers of defi brillation were(1.20 ± 0.63) , and he average number of adrenaline application were (1.20 ± 0.42) times. Neither visible hemorrhage on epicardium nor gross pulmonary congestion was observed. Conclusions The modified transcutaneous electrical stimulation on epicardium to produce CA model in rats is an easily applicable and effective technique. This model may provide an alternative for experimental research of CPR.
