Study of the relationship between brain injury and glucose metabolism in rat model of cardiac arrest
10.3760/cma.j.issn.1671-0282.2018.05.009
- VernacularTitle:大鼠心脏骤停后脑损伤与葡萄糖代谢关系的实验研究
- Author:
Hengjie LI
1
;
Yuanzheng LU
;
Hongyan WEI
;
Yan YANG
;
Chunlin HU
;
Wenwei CAI
;
Hui MAO
;
Xiaoxing LIAO
Author Information
1. 浙江省人民医院 杭州医学院附属人民医院急诊科
- Keywords:
Cardiac arrest;
Positron emission tomography;
Maximal standardized uptake value;
Glucose metabolism;
Neurological defi cit score;
Apoptosis
- From:
Chinese Journal of Emergency Medicine
2018;27(5):502-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the relationship between brain injury and cerebral glucose metabolism in rat model of cardiac arrest. Methods Asphyxia-induced cardiac arrest model was established. Forty-two male Wistar rats were randomly assigned to sham or experimental groups. Rats in the CA4,CA6 and CA8 group were treated with cardiopulmonary resuscitation(CPR) 4 min, 6 min and 8 min after cardiac arrest, respectively. The maximum standardized uptake value (SUVmax) of glucose was detected by PET, and neural defi cit score (NDS) were evaluated at 24 h and 72 h after ROSC. The numbers of injured neurons and apoptotic cells and the protein level of hexokinase I (HXK I) were measured at 72 h after ROSC. Results SUVmax, NDS and the level of HXK I were all decreased after ROSC, and interestingly, this declination of these markers was correlated with the prolongation of the duration of CA, the longer duration of CA the more declination of these biomarkers. Accordingly, the number of injured neurons and apoptotic cells increased were correlated with duration of CA, and thus CA8 group had greater numbers of those cells than CA6 group and CA4 group (P<0.05),and CA6 group had greater numbers of those cells than CA4 group(P<0.05). In addition, the SUVmaxwas positively correlated with NDS(P<0.05), and negatively correlated with the numbers of injured neurons and apoptotic index(P<0.05). Conclusions The degree of brain injury is associated with cerebral glucose metabolism, and PET may become a novel method to assess the severity of brain damage after CA.
