Mesenchymal stem cells transplantation improves functional recovery after cardiac arrest: contribution of necroptosis
10.3760/cma.j.issn.1671-0282.2018.01.008
- VernacularTitle:坏死性凋亡在干细胞移植改善心搏骤停后神经功能的作用
- Author:
Qingming LIN
1
;
Xiahong TANG
;
Shirong LIN
;
Bendun CHEN
;
Feng CHEN
Author Information
1. 福建医科大学省立临床学院 福建省急诊医学研究所 福建省急救中心 福建省立医院急诊科
- Keywords:
Cardiac arrest;
Cardiopulmonary resuscitation;
Mesenchymal stem cells;
Necroptosis;
Neurological deficit scores;
Receptor-interacting protein kinase 1;
Receptor-interacting protein kinase 3
- From:
Chinese Journal of Emergency Medicine
2018;27(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of bone marrow mesenchymal stem cells (MSCs) transplantation on receptor-interacting protein kinase 1 (RIP1) and RIP3 in rat brain after cardiac arrest (CA).Methods Sprague Dawley (SD) rats were randomly (random number) divided into sham group (n=8),CA group (n=8) and MSCs group (n=8).Animals were subjected to asphyxial cardiac arrest and followed by cardiopulmonary resuscitation (CPR).In MSCs group or CA group,animals received intravenous injection of 1 × 106 MSCs in 0.5 mL phosphate buffer solution (PBS) or 0.5 mL PBS alone at 1 h after successful resuscitation.Neurological deficit scores (NDS) were assessed at 3 d after CPR.Donor MSCs in brain were detected under a fluorescent microscope.HE staining of brain tissue was performed to observe necrotic neurons.Western blot analysis was performed to measure the levels of RIP1 and RIP3 in brain.Multiple comparisons were made by analysis of variance or Kruskal-Wallis H test.Results At 3 d after CPR,MSCs group demonstrated higher NDS than CA group [72.5(71.5,73.2) vs.63.0(62.5,64.1),Z=3.376,P=0.001].DAPI-labeled MSCs were primarily observed in the cerebral cortex.The percentage of necrotic neurons in MSCs group was significantly lower than that in CA group [(29.6±5.9)％ vs.(57.2±6.4)％,t=8.922,P＜0.01].The levels of RIP1 and RIP3 expression in brain in MSCs group were significantly lower than those in CA group [RIP1:0.227(0.193,0.243) vs.0.599(0.535,0.629),Z=3.151,P=0.001;RIP3:0.217(0.203,0.274) vs.0.543(0.533,0.555),Z=3.361,P=0.001].Conclusion MSCs transplantation improves neurological function after CPR from CA in rats likely associated with inhibiting necroptosis.
