Preparation and identification of monoclonal antibody against BNTN3 A3
10.7644/j.issn.1674-9960.2018.02.006
- VernacularTitle:BTN3A3单克隆抗体的制备与鉴定
- Author:
Qian LU
1
;
Di LIU
;
Li TANG
Author Information
1. 安徽医科大学研究生院
- Keywords:
butyrophilins;
BTN3A3;
monoclonal antibody;
hybridomas;
immunglobulins
- From:Military Medical Sciences
2018;42(2):105-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and identify monoclonal antibodies against BTN 3A3.Methods BALB/c mice were immunized with the BTN3A3 recombinant protein BTN3A3-mFc containing the mouse IgG2a Fc tag as an antigen, and hybridoma cells were obtained by cell fusion .BTN3A3 recombinant protein BTN3A3-hFc containing a human IgG1 Fc tag as a detection target was used to screen hybridoma cell lines secreting monoclonal antibodies against BTN 3A3.The above monoclonal antibodies were identified with Western blot and indirect immunofluorescence .Results Seven hybridoma cell lines that could stably secret BTN 3A3 monoclonal antibodies were obtained . ELISA, Western blot and indirect immunofluorescence showed that the above antibodies specifically recognized BTN 3A3.Conclusion BTN3A3 monoclonal antibodies that can be applied to ELISA , Western blot or immunofluorescence have been obtained , which can contribute to the functional study of BTN3A3.
