Effect of cadmium on mitochondrial function of hematopoietic stem cells in mouse bone marrow
10.7644/j.issn.1674-9960.2018.02.004
- VernacularTitle:镉对小鼠骨髓造血干细胞线粒体功能的影响
- Author:
Hui HU
1
;
Wei-Li LIU
;
Xue-Lian ZHANG
;
Kun ZHOU
;
Dong YANG
;
Zhi-Qiang CHEN
;
Zhi-Gang QIU
;
Min JIN
;
Zhao-Li CHEN
;
Jun-Wen LI
Author Information
1. 军事科学院军事医学研究院环境医学与作业医学研究所
- Keywords:
cadmium;
mouse model;
hematopoietic stem cells;
mitochondrial dysfunction
- From:
Military Medical Sciences
2018;42(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of cadmium chloride on mitochondrial function of hematopoietic stem cells in mouse bone marrow .Methods After being quarantined for one week , male Kunming mice weighted 20 ±2 g were randomly divided into three groups: control group , low dose cadmium-exposure group and high dose cadmium-exposure group.Mice in low dose and high dose cadmium-exposure groups were exposed to cadmium chloride solution at a dose of 7.5, 15 mg/kg body mass while those in control group were given an equal volume of distilled water through gavage administration every Monday , Wednesday and Friday for six consecutive weeks before cells in mouse bone marrow were collected at the 8th week.Mitochondrial membrane potential and ROS levels of mouse hematopoietic stem cells were detected using a flow cytometry .Results Compared with control group , the gain of body weight was significantly suppressed in cadmium-exposure group (P<0.01).Compared with control group, mitochondrial ROS levels of hematopoietic stem cells significantly increased in cadmium-exposure group and was dose-related(P<0.05,P<0.01). Besides, mitochondrial membrane potential of hematopoietic stem cells decreased in cadmium -exposure group compared with control group and was dose-related(P<0.05,P<0.01).Conclusion Cadmium exposure can lead to dose-related mitochondrial dysfunction of hematopoietic stem cells via oxidative damage in Kunming mice .
