Evaluation of activity of botulinum neurotoxin A based on primary cerebellar granu-losa cells
10.7644/j.issn.1674-9960.2018.01.014
- VernacularTitle:基于原代小脑颗粒细胞的A型肉毒毒素活性分析法的建立
- Author:
Jian-Xin WANG
1
;
Zhan LI
;
Zhi-Li HE
;
Jie HUANG
;
Tao LI
;
Hui WANG
Author Information
1. 军事科学院军事医学研究院微生物流行病研究所
- Keywords:
botulinum neurotoxin A;
primary cerebellar granulosa cells;
activity analysis
- From:
Military Medical Sciences
2018;42(1):57-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and culture the primary cerebellar granulosa cells(CGNs)of SD rats and evaluate the activity of botulinum neurotoxin A(BoNT/A)based on CGNs.Methods CGNs of 6-to 8-days-old SD rats were isolated and cultured.After 5-7 d,the cells were treated with BoNT/A.The activity of the toxin was evaluated with immunofluo-rescence,and the relationships between the activity and dose of the toxin were analyzed with Western blotting.Results and Conclusion CGNs Of SD rats were successfully cultured,a method for evaluating the activity of BoNT/A was established at the level of primary nerve cells,and the relationships between the activity and dose of toxin were analyzed.This study provides a tool for further detailing the biochemical mechanism of BoNT /A.
