Evaluation of target-enrichment accompanied by next-generation sequencing for detection of four pathogens in bloodstream infections
10.7644/j.issn.1674-9960.2018.01.013
- VernacularTitle:检测4种血流感染病原体的靶向序列富集与测序相结合技术方法评价
- Author:
Qian LI
1
;
Peng LIU
;
Yan ZHANG
;
Chao ZHANG
;
Wen-Hua HUANG
;
Yu-Ling ZHENG
;
Hong-Quan WANG
;
Yong-Qiang JIANG
Author Information
1. 军事科学院军事医学研究院微生物流行病研究所
- Keywords:
bloodstream infection;
Ampliseq;
the next-generation sequencing;
target-enrichment
- From:
Military Medical Sciences
2018;42(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an Ampliseq method that combines target-enrichment and the next-generation sequencing for simultaneous detection of Staphylococcus aureus, S.epidermidis, Klebsiella pneumoniae and Listeria monocytogenes in order to provide a fast and accurate means to detect pathogens in bloodstream infections.Methods A method to evaluate the LOD,specificity and sensitivity by constructing simulated samples spiked in known pathogens was established.Results and Conclusion Target-enrichment Ampliseq showed good sensitivity and specificity,and the limit of detection(LOD)was as low as 101CFU/ml.The sensitivity was 95.38%,the specificity was 95.45%and the Kappa value was between 0.839 -1.000.This method can detect S.aureus,S.epidermidis,K.pneumoniae and L.monocytogenes simultaneously in one reaction within 15 hours.
