Protective effects and possible mechanism of 12-O-tetradecanoylophorbol-13-decano-ate against acute intestinal radiation injury of mice
10.7644/j.issn.1674-9960.2018.01.012
- VernacularTitle:12-氧-十四烷酰佛波醇-13-癸酰酯对小鼠肠道急性放射损伤的保护作用及机制研究
- Author:
Xi LIU
1
;
Zhi-Hong WANG
;
Hong-Wei ZHOU
;
Yi-Bing YAO
;
Mo LIU
;
Min-Hang ZHOU
;
Li CHENG
;
Jun-Zhong SUN
Author Information
1. 锦州医科大学解放军总医院第一附属医院研究生培养基地
- Keywords:
12-O-tetradecanoylophorbol-13-decanoate(TPD);
mice;
radiation injuries;
intestine;
small;
acute intestinal radiation injury;
intestinal crypt;
radiotherapy;
Gamma rays;
protection mechanism
- From:
Military Medical Sciences
2018;42(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 12-O-tetradecanoylophorbol-13-decanoate(TPD)on protection against acute intestinal radiation injury of mice and the possible mechanism.Methods Twenty female BALB/c mice aged 6-8 weeks were divided by random number table method into the control group and TPD groups(25,50,and 100 μg/kg). A radiation-damaged model of mice was irradiated by 10 Gy 60Co γ-rays,while the TPD groups were pretreated for 3 d with caudal vein injection before irradiation.The survival time of 20 days and the number of crypts at 3.5 days after irradiation were detected.Rat intestinal epithelial cells(IEC-6)were treated with 1 nmol/L TPD for 12 h before irradiation with 10 Gy 60Co γ-rays,and CCK-8 was used to detect the capability of cell proliferation at 0,1,2,3 and 4 d after irradiation. Results The mice in the control group survived for an average of 4.2 days,compared to 10 days in the optimal TPD group (100 μg/kg).The average number of crypts in the control group and the best TPD group was 11.0 ±1.3 and 35.1 ±1.9 respectively.The proliferation activity of IEC-6 was measured for four consecutive days.The average D value of the TPD groups was significantly higher than that of control.Conclusion TPD has a protective effect against acute intestinal radiation injury, and its protective mechanism may be achieved by promoting intestinal crypt cell proliferation and increasing the number of crypts in the intestine.
