Construction of BRCC3 gene knockout mice and preliminary analysis of the pheno-type
10.7644/j.issn.1674-9960.2018.01.011
- VernacularTitle:BRCC3基因敲除小鼠的构建和表型初步分析
- Author:
Yang XIAO
1
;
Guang-Ming REN
;
Yi-Qun ZHAN
;
Miao YU
;
Chang-Yan LI
;
Xiao-Ming YANG
;
Rong-Hua YIN
Author Information
1. 军事科学院军事医学研究院
- Keywords:
BRCC3;
mice,knockout;
phenotype;
genotype;
gene expression;
ubiquitination
- From:Military Medical Sciences
2018;42(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct BRCC3(BRCA1/BRCA2-containing complex subunit 3)gene knockout mice and preliminarily study the phenotypes.Methods Using the Cas9/sgRNA-Mediated genome Editing, the BRCC3 knockout mouse models were constructed.Genomic DNAs of mouse tail tissues were extracted and identified, the genotypes of mice were determined at the DNA level,and RNAs and proteins of tissues, such as the heart, liver, spleen, lung, kidney of mice were extracted and the expression of BRCC3 gene was detected by real-time-PCR and Western blotting(WB).The trend of relative body mass change and indexes that might affect the growth development and metabolism were observed. Major organs were hematoxylin-eosin(HE)stained and observed.The routine blood test of peripheral blood of mice was conducted.Results The mouse model of BRCC3 knockout was successfully constructed.BRCC3 knockout mouse survived and were fertile, indexes of blood lipid and liver function were normal, organs were not degenerative and indexes of peripheral blood in routine blood test were all in the normal range.The relative body mass of BRCC3 knockout mice was higher than that of wild type mice,and the level of serum cholesterol was increased.Conclusion BRCC3 may be involved in relative body mass regulation and cholesterol metabolism in mice.
