Role of Abro1 in LPS-induced acute lung injury
10.7644/j.issn.1674-9960.2017.12.002
- VernacularTitle:Abro1在LPS诱导的急性肺损伤中的作用探讨
- Author:
Jiao-Jiao CHEN
1
;
Fei-Fei SUN
;
Yi-Qun ZHAN
;
Chang-Hui GE
;
Xiao-Ming YANG
Author Information
1. 广东药科大学
- Keywords:
respiratory distress syndrome,adult;
ALI;
LPS;
mice;
knockout;
Abro1;
bronchoalveolar lavage fluid
- From:Military Medical Sciences
2017;41(12):952-955,961
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Abro1 on acute respiratory distress syndrome(ARDS)/acute lung injury(ALI)in mice.Methods Abro1 knock-out(KO)mice and wild type(WT)mice were both randomly divided into two groups for intratracheal instillation of lipopolysaccharide(LPS)or normal saline.At 6 or 24 hours after treatment, the pathological changes in lung tissue were observed by HE staining.At 6 hours after treatment,inflammatory immune cells and cytokines production(IL-6)in the bronchoalveolar lavage fluid were examined.Myeloperoxidase(MPO)and the mRNA level of IL-6 in the lung tissue were compared.Results At 24 hours after treatment, compared with WT mice treated with LPS,Abro1 KO mice showed a significantly lower lung injury score.At 6 hours after treatment,Abro1 depletion resulted in reduced levels of inflammatory immune cell infiltration and cytokines production(IL-6)in the bronchoalveolar lavage fluid(P<0.05).In addition,the MPO content and the mRNA level of IL-6 in the lung tissue were much lower than those in WT mice treated with LPS for 6 hours(P<0.05).Conclusion Abro1 deficiency can attenuate LPS-induced ARDS/ALI.
