Ubiquitin ligase Smurf1 promotes poly-ubiquitination of PDK1
10.7644/j.issn.1674-9960.2017.12.001
- VernacularTitle:泛素连接酶Smurf1促进PDK1的多聚泛素化修饰
- Author:
Jun WEI
1
;
Hui-Sen SHI
;
Zhi-Sheng DANG
;
Ling-Qiang ZHANG
;
Xue-Li ZHANG
Author Information
1. 锦州医科大学奉贤中心医院研究生培训基地
- Keywords:
ubiquitin ligases;
ubiquitination;
immunoprecipitation;
immunoblotting;
Smurf1;
PDK1;
PDPK1;
cell proliferation;
plasmids;
mutation
- From:
Military Medical Sciences
2017;41(12):947-951
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of 3-phosphoinositide-dependent protein kinase 1(PDK1)poly-ubiquitination.Methods Co-immunoprecipitation(Co-IP)and Western blot(WB)were used to analyze poly-ubiquitination of PDK1.It was confirmed that ubiquitin ligase smad ubiquitylation regulatory factor 1(Smurf1)inprove PDK1 poly-ubiquitination within MEF cells,site-directed mutagenesis and WB before PDK1 poly-ubiquitination sites were determined.Results We found that PDK1 could undergoes poly-ubiquitination,ubiquitin ligase Smurf1 was found to be a direct E3 ligase for PDK1 poly-ubiquitination and might rely on the ubiquitin ligase Smurf 1 K699 site activity.K304 was PDK1 poly-ubiquitination modification site point.Conclusion The ubiquitin ligase Smurf1 can promate poly-ubiquitination of PDK1.
