An area CCD-based detection system for up-converting phosphor immune lateral flow assay
10.7644/j.issn.1674-9960.2017.11.009
- VernacularTitle:基于面阵CCD的便携式上转换发光免疫层析检测系统
- Author:
Chao LI
1
;
Zi-Jiang YANG
;
Yao-Hua DU
;
Tai-Hu WU
;
Feng CHEN
Author Information
1. 军事科学院系统工程研究院卫勤保障技术研究所
- Keywords:
area CCD;
up-converting phosphor;
immune chromatography;
biosensing techniques;
laser uniformity
- From:Military Medical Sciences
2017;41(11):906-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To make portable the detector for up-converting phosphor (UCP) immune lateral flow assay portable and increase the test precision by developing an area CCD-based detection system for UCP immune lateral flow assay.Methods The excitation light source was a 980 nm and 300 mW laser diode.In order to decrease the error induced by the un-uniformity of laser irradiation,a uniformity mirror was inserted in the beam and a calibration algorithm was optimized.The residual error from the un-uniformity irradiation was less than 1％.Results Thanks to the adjustability of the exposure time,the dynamic range of detection of the system was as high as 106 dB.The repeat test error for the very low signal was 1％ (variation coefficient).The linear correlation coefficient between the tested T/C value and sample concentration was 0.998.Conclusion Compared to is traditional instrument,this detection system is static,portable and highly precise.
