Effects of sleep deprivation on spleen circadian clock gene expression in rats
10.7644/j.issn.1674-9960.2017.11.006
- VernacularTitle:睡眠剥夺对大鼠脾脏淋巴细胞生物钟基因表达水平的影响
- Author:
Chen XING
1
;
Ye GU
;
Xiu-Duan XU
;
Shu-Xian ZOU
;
Yong-Liang HU
;
Mei-Ru HU
;
Lun SONG
Author Information
1. 军事科学院军事医学研究院军事认知与脑科学研究所神经免疫与抗体工程研究室
- Keywords:
sleep deprivation;
circadian rhythm;
circadian clock;
gene expression;
lymphocytes
- From:Military Medical Sciences
2017;41(11):894-897
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 72 h sleep deprivation (SD) on circadian clock gene expression in the rat spleen.Methods The rats were randomly divided into control group and SD group.An SD instrument was used to deprive the rats of sleep for 72 h.Then the lymphocytes from the spleen were obtained by Ficoll seperation medium before the expression of clock genes was detected by RT-PCR and Western blotting analysis respectively.Results Compared with the control group,the mRNA levels of bmal1,clock,per2 and rev-erbα strikingly decreased in the spleens of the SD group rats.However,npas2,per1,rorα and cry1 mRNA expression hardly changed in the control and SD group.Meanwhile,the protein levels of spleen BMAL1,NPAS2,CRY1 and RORα were significantly down-regulated and PER1 protein levels were up-regulated in SD group compared with control group.However REV-ERBα protein expression remained unchanged in the control and SD group.Conclusion 72 h SD can result in abnormal expressions of several circadian clock genes in lymphocytes of the spleen at both transcription and translation levels.
