The establishment of portal hypertension model in mice
10.3969/j.issn.1008-794X.2018.03.012
- VernacularTitle:门静脉高压症小鼠模型构建
- Author:
Liteng LIN
1
;
Mingyue CAI
;
Wensou, HUANG
;
Jingjun HUANG
;
Tian LAN
;
Kangshun ZHU
Author Information
1. 510260,广州医科大学附属第二医院微创介入科
- Keywords:
portal hypertension;
bile duct ligation;
carbon tetrachloride;
mouse model
- From:
Journal of Interventional Radiology
2018;27(3):242-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish two types of portal hypertension (PHT) models in mice by using bile duct ligation (BDL) method and carbon tetrachloride (CCl4) induction technique respectively. Methods A total of 24 C57BL/6 mice were randomly and equally divided into the following four groups with 6 mice in each group: group BDL, control group of BDL, group CCl4, and control group of CCI4. After the establishment of PHT, the main portal vein was punctured in all experimental mice to measure the portal vein pressure, and blood sampling was collected to test serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Using hematoxylin eosin (HE) and sirius red staining the liver tissues were pathologically examined. Immunohistochemical study of alpha smooth muscle actin (SMA) was adopted to evaluate the liver function, hepatic fibrosis and hepatic stellate cell activation status. Results Both modeling methods could make the portal vein pressure increased in experimental mice. The increasing of portal vein pressure in group CCl4 was more obvious. Compared with their corresponding control groups, the degree of liver damage, hepatic fibrosis and hepatic stellate cell activation in group BDL and group CCl4 were more serious. Conclusion Both BDL method and CCl4 induction technique can successfully establish the mouse model of PHT. All the portal venous pressure, the serum biochemical indices and the changes of liver pathology of the mouse model are well in line with the characteristics of PHT in human. (J Intervent Radiol, 2018, 27:242-246)
