Establishment of cerebral venous sinus thrombosis model in rabbits by using ferric chloride combined with thrombin injection
10.3969/j.issn.1008-794X.2018.02.013
- VernacularTitle:氯化铁结合凝血酶构建兔静脉窦血栓模型
- Author:
Ying WEI
1
;
Sheng GUAN
;
Xinbin GUO
;
Xin DENG
;
Zibo WANG
;
Dongdong LI
;
Xiaoke LU
;
Yanhua DONG
Author Information
1. 450052,郑州大学第一附属医院神经介入科
- Keywords:
cerebral venous sinus thrombosis;
animal model;
thrombus recanalization;
pathological change
- From:
Journal of Interventional Radiology
2018;27(2):151-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the establishment of cerebral venous sinus thrombosis (CVST) model in rabbits by local application of ferric chloride at sinuses sagittalis superior (SSS) combined with thrombin injection, and to evaluate its feasibility and application value. Methods A total of 39 New Zealand white rabbits were randomly and equally divided into three groups with 13 rabbits in each group, local application of cotton piece saturated with saline at SSS for 10 minutes was performed for the rabbits of group A, SSS local application of cotton piece saturated with 40％ ferric chloride for 10 minutes was adopted for the rabbits of group B, while SSS local application of cotton piece saturated with 40％ ferric chloride for 5 minutes together with injection of thrombin was carried out for the rabbits of group C. Whole cerebral DSA was performed immediately after modeling to judge if there was formation of thrombosis. Two days after the modeling, every 3 rabbits from each group were sacrificed to make 2, 3, 5-chloride triphenyl tetrazole (TTC) staining. Seven days after the modeling, the remaining 10 rabbits of each group were examined with DSA, the vascular recanalization rates were calculated, and the histopathological examination was made. Results In group B and group C, SSS thrombosis with surrounding cerebral infarction, edema, inflammatory cell aggregation and other pathological changes were observed. The 7-day vascular recanalization rate in group C was strikingly lower than that in group B (10％ vs 70％, P＜0.05). Surrounding cortical vein thrombus and subcortex petechial hemorrhages were obviously seen in group C. Conclusion For the establishment of CVST model in rabbits, local application of ferric chloride at SSS together with thrombin injection is effective and feasible. The thrombus thus induced is quite stable, and its pathogenesis and pathophysiology are quite similar to clinical manifestations. Therefore, this method can be used for basic research and clinical trials of CVST.
