The effect of sorafenib on the expressions of B7-H3 and B7-H4 proteins in different human hepatocellular carcinoma cell lines
10.3969/j.issn.1008-794X.2018.01.014
- VernacularTitle:索拉非尼对不同人肝癌细胞株中B7-H3和B7-H4蛋白表达的影响
- Author:
Han XU
1
;
Shi ZHOU
;
Lizhou WANG
;
Tianzhi AN
;
Tianpeng JIANG
;
Jie SONG
;
Xing LI
Author Information
1. 550004贵阳,贵州医科大学附属医院介入科
- Keywords:
hepatocellular carcinoma cell;
B7-H3 protein;
B7-H4 protein;
Western blotting;
MTT assay
- From:Journal of Interventional Radiology
2018;27(1):58-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of sorafenib on the expressions of B7-H3 and B7-H4 proteins in different human hepatocellular carcinoma cell lines,including HepG2,Hep3B,BEL-7402,BEL-7404,BEL-7405,QGY-7701,QGY-7703,SMMC-7721,MHCC97H,MHCC97L,HCCLM3 and HCCLM6.Methods Western blotting and MTT assay were used to check the influence of sorafenib on the expressions of B7-H3 and B7-H4 proteins in different human hepatocellular carcinoma cell lines and to test the inhibitory effect of sorafenib on different human hepatocellular carcinoma cell lines.Results Compared with normal human liver cells (HL-7702),the expressions of B7-H3 and B-H4 proteins in different human hepatocellular carcinoma cell lines were significantly up-regulated (P＜0.01).The cytotoxic activity IC50 values of sorafenib to Hep3B,BEL-7404,MHCC97H,HCCLM3 and HCCLM6 were 14.56,9.14,9.46,17.21 and 9.29 μmol/L respectively.After treating Hep3B,BEL-7404,MHCC97H,HCCLM3 and HCCLM6 with sorafenib at the doses of 5,10 and 20 μmol/L separately,the expressions of B7-H3 and B7-H4 proteins were strikingly down-regulated when compared with the control group (P＜0.01).Conclusion The overexpressions of B7-H3 and B7-H4 proteins in different human hepatocellular carcinoma cell lines are a common finding,which can influence tumor immune escape.It may be a new target for prevention and treatment of liver cancer in future.
