Expression of hsa_circ_PVT1 in human hepatocellular carcinoma and its clinical significance
10.11855/j.issn.0577-7402.2018.03.06
- VernacularTitle:Hsa_circ_PVT1在肝细胞癌中的表达及意义
- Author:
Yuan-Xin ZHU
1
;
Hang-Wei FU
;
Xia LIN
;
Xiang LAN
;
Yu MA
;
Guang-Yao LI
;
Rui DONG
;
Yu-Zhou WANG
;
Ping CHEN
Author Information
1. 400042,重庆 陆军军医大学大坪医院野战外科研究所肝胆外科
- Keywords:
hepatocellular carcinoma;
circular RNA;
PVT1;
cell proliferation;
cell cycle
- From:
Medical Journal of Chinese People's Liberation Army
2018;43(3):211-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression and clinical significance of circ-PVT1 in human hepatocellular carcinoma (HCC) and its effect on HCC cell proliferation.Methods The expressions of circ-PVT1 in hepatocellular carcinoma and the matched tumor-adjacent tissues were detected by RT-qPCR and the relationship between pathological indexes and the expression level was analyzed in 46 patients.The expressions of circ-PVT1 in human normal liver cell line (L02) and hepatocellular carcinoma cell lines (HepG2,SMMC-7721,MHCC-97H,MHCC-97L,HCC-LM3) were detected by RT-qPCR and were compared thereafter.With knocking down the expression of circ-PVT1,si-circPVT1 was transfected into HepG2 and SMMC-7721 cells by using lipofectamine technique in vitro,with the si-NC being taken as negative control.After interfering the expression of circ-PVT1,the effect on the proliferation of hepatocellular carcinoma cells was detected by CCK-8 and EDU experiments and flow cytometry was conducted to observe the effect of circ-PVT1 on cell cycle.Results The expression level of circ-PVT1 was significantly higher in HCC tissues than in adjacent tissues (P＜0.01),and its high expression level was significantly correlated with tumor size,TNM stage and differentiation degree.Similarly,in human hepatocellular carcinoma cell lines (HepG2,SMMC-7721,MHCC-97H,MHCC-97L,HCC-LM3),the expression level of circ-PVT1 was also higher than that in human normal liver cell line L02 (P＜0.05).Compared with the negative control group,silencing of circ-PVT1 resulted in remarkable reduction in cell proliferation of HepG2 and SMMC-7721.Conclusion circ-PVT1 may act as a potential biomarker for HCC diagnosis and may become a novel proliferation factor.
