Effect of chronic intermittent hypoxia on glycometabolism in rat' liver and the mechanism thereof
10.11855/j.issn.0577-7402.2018.03.05
- VernacularTitle:慢性间歇性缺氧对大鼠肝脏糖代谢的影响及机制
- Author:
Wei YU
1
;
Ze-Ping QIN
;
Hua-Jiao CHEN
;
Feng ZHANG
;
Bing LI
Author Information
1. 400037,重庆 陆军军医大学第二附属医院耳鼻喉科
- Keywords:
chronic intermittent hypoxia;
insulin resistance;
glucose transporter 2;
insulin receptor substrate 2;
leptin;
free fatty acid
- From:
Medical Journal of Chinese People's Liberation Army
2018;43(3):206-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of chronic intermittent hypoxia on the adipose factor and the expressions of insulin receptor substrate 2 (IRS-2),glucose transporter 2 (GLUT-2) and leptin in rat liver.Methods Twenty-four mature SD rats were randomly divided into 3 groups:control group (UC),chronic intermittent hypoxia group (CIH) and reoxygenation group (RH).The arterial blood gas analysis was performed after the establishment of rat model.The serum fasting blood glucose (FBG) and fasting insulin (FINS) in each group were detected by peroxidase method;the concentrations of free fatty acids (FFA) and leptin were detected by ELISA.The expressions ofmRNA and protein of GLUT-2,IRS-2 and leptin were detected by qRT-PCR and Western blotting.Results The serous concentrations of FBG,FINS,FFA and leptin were significantly higher in CIH group than in UC group (P＜0.05),and were dramatically higher in RH group than in both CIH group (P=0.003) and UC group (P=0.000).Western blotting and qRT-PCR detection showed that the protein and mRNA expressions of GLUT-2 and IRS-2 were significantly lower in CIH group than in RH group of rat liver (P＜0.05),while were markedly lower in RH group than in UC group (P＜0.05);the expressions of leptin protein and mRNA were significantly higher in CIH group than in RH group (P＜0.05),while were obviously higher in RH group than in UC group of rat liver (P＜0.05).Conclusion Insulin resistance induced by chronic intermittent hypoxia may be associated with the elevation of serum FFA and leptin,and be related to the decreased expression of GLUT-2 and IRS-2 and increased expression of leptin in liver.
