Diagnostic value of interferon-γ release assay of peripheral blood and pleural effusion for tuberculous pleurisy
10.11855/j.issn.0577-7402.2018.01.11
- VernacularTitle:外周血及胸腔积液γ-干扰素释放试验对结核性胸膜炎的诊断价值
- Author:
Min ZHOU
1
;
Qing-Luan YANG
;
Hua-Xin CHEN
;
Zhi-Ming YU
;
Liang GAO
;
Qian-Qian LIU
;
Qin-Fang OU
Author Information
1. 214005江苏无锡,无锡市第五人民医院肺科
- Keywords:
interferon-γ release assay;
T-SPOT.TB;
pleural effusion;
tuberculous pleurisy;
diagnosis
- From:
Medical Journal of Chinese People's Liberation Army
2018;43(1):56-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of interferon-γ release assay of blood and pleural effusion for tuberculous pleurisy.Methods Fifty-six adult patients with suspected tuberculous pleurisy were enrolled in our study.The blood and pleural effusion interferon-γ release assay were measured by T-SPOT.TB test in 38 pleural tuberculosis patients and 18 nontuberculous pleurisy controls.The diagnostic sensitivity,specificity,predictive value of T-SPOT.TB in pleural effusion mononuclear cells (PE-MC) and peripheral mononuclear cells (PBMC) were analyzed.Results The sensitivities and specificities,positive predictive values and negative predictive values,respectively,of the PE-MC and PBMC for diagnosing were as follows:86.5％(95％ confidence interval[CI] 71.2％-95.5％) and 100％(95％CI 90.5％-100％);52.9％(95％CI 27.8％-77.0％) and 35.3％(95％CI 14.2％-61.7％);80.0％(95％CI 64.4％-90.9％) and 77.1％(95％CI 62.7％-88.0％);64.3％(95％CI 35.1％-87.2％) and 100％(95％CI 54.1％-100％).By ROC curve analysis,a cut-off value of 47SFC/2.5 × 105 cells in PE-MC showed a sensitivity of 89.2％ and a specificity of 88.2％.Conclusion T-SPOT.TB in PE-MC could be an accurate diagnostic method for tuberculous pleurisy in TB endemic settings.Moreover,47SFC/2.5 × 105 cells might be the optimal cut-off value for diagnosing tuberculous pleurisy.
