Analysis of serum peptides in Candida albicans bloodstream infection based on MALDI-TOF MS
10.11855/j.issn.0577-7402.2018.01.05
- VernacularTitle:应用基质辅助激光解吸电离飞行时间质谱研究白色念珠菌血流感染的血清多肽指纹图谱
- Author:
Ya-Ting MA
1
,
2
;
Ming YANG
;
Shang HE
;
Chen CHEN
;
Cheng-Bin WANG
Author Information
1. 100853北京 解放军总医院临床检验科
2. 325035浙江温州 温州医科大学检验医学院生命科学学院
- Keywords:
matrix-assisted laser desorption/ionization time of flight mass spectrometry;
bloodstream infection;
Candida albicans;
serum peptide profiling
- From:
Medical Journal of Chinese People's Liberation Army
2018;43(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the fingerprints of serum peptides in bloodstream infection induced by Candida albicans (C.albicans),with matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS),and establish the corresponding diagnostic model Methods To establish ICR mice model of C.albicans bloodstream infection,and collect the serum samples which were purified by weak cation exchange beads.The serum peptide finger printing was recognized by MALDITOF MS,and BioExplorer software was used for analysis between infection group and normal control group.Furthermore,the peptide fingerprints were analyzed between C.albicans infection group,Escherichia coli (E.coli) infection group and Staphylococcus aureus (S.aureus) infection group.Results Comparison between C.albicans infection group and normal control group,there were 135 polypeptide peaks,of which 5 polypeptides up-regulated,6 down-regulated and 7 up-regulated first and down-regulated afterwards.The diagnostic model was established by combination of 6 peaks (i.e.m/z 1610.9,1742.3,2666.4,2778.0,3345.1 and 4528.8),which possessed an accurate rate of 80.0％ in diagnosis of C.albicans infection.It was found by comprehensive comparison between C.albicans,E.coli and S.aureus infection groups that there were 5 polypeptides expressed collectively,i.e.m/z1513.8,2910.8,3538.1,3884.9 and 5007.3.Conclusions MALDI-TOF MS can be used to distinguish the C.albicans infection and normal polypeptide peaks.The collective polypeptides expressed in the infection groups can be further used to diagnose the infection.Establishment of corresponding diagnostic models may be helpful for early diagnosis of fungal bloodstream infection and provide a basis for reasonable clinical medication.
