Early detection of the bloodstream infection bacteria based on MALDI-TOF MS
10.11855/j.issn.0577-7402.2018.01.04
- VernacularTitle:基于基质辅助激光解吸电离飞行时间质谱系统的血流感染致病菌早期检测
- Author:
Ke-Xin ZHANG
1
,
2
;
Chong HU
;
Chen CHEN
;
Shang HE
;
Ming YANG
;
Cheng-Bin WANG
Author Information
1. 325000浙江温州 温州医科大学检验医学院/生命科学学院
2. 100853北京 解放军总医院临床检验科
- Keywords:
Blood stream infection bacteria;
Early detection;
High-throughput detection
- From:
Medical Journal of Chinese People's Liberation Army
2018;43(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a clinical method for early detection of the bloodstream infection bacteria based on matrix-assisted laser ionization time of flight mass spectrometry (MALDI-TOF MS).Methods After consulting the Laboratory Information System statistics and domestic related literature,We chose 10 kinds of bacteria (Escherichia coli.,Pseudomonas aeruginosa,Acinetobacter baumannii,Klebsiella pneumoniae,Enterococcus faecium,Enterococcus faecalis,Staphylococcus aureus and Staphylococcus epidermidis,Proteus mirabilis,Streptococcus pneumoniae) as target.The MALDI-TOF MS was established using simulated bacterial infection blood samples.From March to May 2017,33 blood samples of suspected sepsis patients from Emergency Department,General Hospital of PLA,were tested.Results The MALDI-TOF MS whose detecting sensitivity was 100CFU/ml had the same negative detection rate with the blood culture (27cases/27cases,100％).Besides the 2 samples of Morganella morganii infection and Staphylococcus hominis infection were out of the range,the results of the remaining 4 positive samples were consistent (100％).Conclusion Compared to the blood culture and biochemical identification,MALDI-TOF MS can rapidly detect 10 kinds of bloodstream infection bacteria with high sensitivity and high accuracy.
