Effects of protein PUMA-α on the apoptosis and fibrosis of human peritoneal mesothelial cells induced by high glucose
10.11855/j.issn.0577-7402.2017.09.06
- VernacularTitle:PUMA-α蛋白在高糖诱导的人腹膜间皮细胞凋亡及纤维化中的作用
- Author:
Ming-Wen CHE
1
;
Feng-Rong LI
;
Li-Fang GONG
;
Xiu-Feng FANG
;
Hai-Ping DENG
;
Xi JIANG
;
Ya-Wei TANG
;
Han-Min WANG
Author Information
1. 841000,新疆库尔勒 解放军273医院内三科
- Keywords:
apoptosis;
PUMA-α protein;
human peritoneal mesothelial cells
- From:
Medical Journal of Chinese People's Liberation Army
2017;42(9):781-787
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of p53 up-regulated modulator of apoptosis (PUMA)-α protein on the apoptosis and fibrosis of human peritoneal mesothelial cells (HPMCs) induced by high glucose.Methods HPMCs were induced by 50mmol/L D type glucose or mannitol for 72 hours respectively,flow cytometry was employed to detect the rate of apoptotic cells,and theexpression levels of apoptosis-and fibrosis-related proteins were detected by Western blotting.The untreated HPMCs were transfected with Lenti-PUMA-α,and the treated cells were transfected with shRNA-PUMA-α,the number of apoptotic cells and the expression levels of apoptosis-and fibrosis-related proteins were detected with the methods mentioned above.Results Flow cytometry showed that the rate of apoptotic HPMCs increased after being induced by high glucose for 72 hours,and Western blotting revealed that the expression levels of pro-apoptotic and pro-fibrotic related proteins increased,but the arrestins of apoptosis and fibrosis-related proteins decreased.Up-regulation of PUMA-α promoted apoptosis and fibrosis,while down-regulation of PUMA-α alleviated apoptosis and fibrosis of HPMCs.Conclusion High glucose may accelerate apoptosis and fibrosis of HPMCs by up-regulating the expression of PUMA-α.
