Etomoxir strengthens the inhibitory effect of cisplatin on lung cancer cell line A549
10.3969/j.issn.1001-6325.2018.07.018
- VernacularTitle:乙莫克舍增强顺铂对肺癌细胞系A549的抑制作用
- Author:
Ming-Cheng SONG
1
;
Yan LIU
;
Hui-Wen HE
;
Hui-Lin CHEN
;
Chen ZHANG
;
Chong CHEN
;
Yun-Ping LUO
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 免疫学系
- Keywords:
cisplatin;
etomoxir;
lung cancer;
tumor therapy;
CPT1
- From:
Basic & Clinical Medicine
2018;38(7):993-998
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the combination of carnitine palmityl transferase 1( CPT1) inhibitor etomoxir and chemotherapy drug cisplatin on lung cancer cell line A549 proliferation and migration of lung cancer. Methods CCK8 was applied to investigate the effect of the combination of etomoxir and cisplatin on A549 survival. Transwell test was used to detect migration of lung cancer cell line A549 treated with the combination of etomoxir and cisplatin. Apoptosis of A549 was detected by annexin V/PI. A549 transplanted mice were used to test therapeu-tic effect of etomoxir combined with cisplatin. Results Tumor cells proliferation and migration were inhibited by the combination of etomoxir and cisplatin. Apoptosis of tumor cells was enhanced by the combination of etomoxir. After the treatment of the combination of etomoxir and cisplatin, tumor growth of mouse was inhibited. Conclusions The combination of etomoxir and cisplatin can decrease tumor cells survival and induce tumor cells apoptosis, and then inhibit tumor growth in mouse lung cancer model.
