Sympathetic excitation and dendritic cell activation aggravates myocardial injury in LPS-induced rats
10.3969/j.issn.1001-6325.2018.07.014
- VernacularTitle:交感神经兴奋与树突状细胞活化加剧脂多糖诱导大鼠心肌损伤
- Author:
An-Lei LIU
1
;
Ju-Yuan LIU
;
Jun XU
;
Ji-Hai LIU
;
Hua-Dong ZHU
;
Xue-Zhong YU
Author Information
1. 中国医学科学院 北京协和医学院 北京协和医院 急诊科
- Keywords:
sympathetic excitation;
dendritic cell;
LPS;
myocardial injury
- From:
Basic & Clinical Medicine
2018;38(7):973-977
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sympathetic excitation and dendritic cell activation on myocar-dial injury in LPS-induced rats. Methods The rats were randomly divided into four groups:control group, LPS group ( intraperitoneal injection of LPS 10 mg/kg) ,intervention group ( given beta receptor blocker Ate 5 mg/kg after LPS administration) and DC inhibitor VAG539 intervention group ( VAG539 30 mg/kg gavage twice a day for 2 days after LPS administration) respectively . The powerlab system was used to record the hemodynamic and sympathetic data. The concentration of norepinephrine ( NE) in plasma was measured by high performance liquid chromatography ( HPLC) , and the expression and positive cells of TNF-α and DCs in myocardium were detected by immunohistochemistry. Results Compared with the control group, the plasma NE level significantly increased ( P＜0.05) ;The expression of TNF-α and DCs in heart tissue significantly increased ( P＜0.05) ;the renal sym-pathetic nerve activity (SNA) significantly increased in the three groups after LPS administration for 24 hours. Compared with the LPS group, the plasma NE level significantly decreased ( P＜0.05 ) ; The expression of TNF-α and DCs in heart tissue significantly decreased ( P＜0.05) ;the renal SNA significantly decreased after Ate and VAG539 administration(P＜0.05).Conclusions Excessive activation of sympathetic nervous system and activation of DC aggravate myocardial injury in LPS-induced rats.
