LncRNA Neat1 is upregulated in P19 cell line processed with all-trans-retinoic acid
10.3969/j.issn.1001-6325.2018.07.011
- VernacularTitle:全反式维甲酸诱导P19细胞系长非编码RNA Neat1基因表达
- Author:
Xue-Zhe FAN
1
;
Jing WANG
;
Yan-Jun ZHANG
;
Ye ZHANG
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院
- Keywords:
Neat1;
neural differentiation;
TSA
- From:
Basic & Clinical Medicine
2018;38(7):957-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Neat1 in the differentiation of P19 cells induced by all-trans-retinoic acid(atRA) and to explore the effect of histone modification on its expression. Methods The differentiation of P19 cells was induced with the α-MEM culture media containing 0.5 μmol/L all-trans-retinoic( atRA) acid and the expression Mash1 which is the neural differentiation maker gene was measured. The expression of Neat1 was measured with RT-qPCR. The cells were treated with TSA, NAM or AdOx respectively to investigate the effect of the histone modifier inhibitor on the expression of Neat1. Results The model of differentiation of P19 cells induced by atRA was successfully constructed. Mash1 was significantly upregulated in the process of P19 cells differentiation.Neat1 was significantly upregulated with the induction of P19 cells treated with atRA(P<0.01). The TSA but not the NAM or AdOx could induce the expression of the Neat1.Conclusions The expression of Neat1 is significantly upregulated in the process of P19 differentiation induced by atRA and the the high level of histone acetylation maintained by TSA potentially induce the expression of Neat1.