IFNα induces cytidine/uridine monophosphate kinase 2 expression in hepatocarcinoma cell line to activate macrophages of mice
10.3969/j.issn.1001-6325.2018.07.009
- VernacularTitle:IFNα上调人肝癌细胞系胞苷单磷酸激酶2表达激活小鼠巨噬细胞系
- Author:
Li-Qin CHENG
1
;
Zhu-Qin ZHANG
;
Hou-Zao CHEN
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 医学分子生物学国家重点实验室
- Keywords:
hepatocellular carcinoma;
cytidine/uridine monophosphate kinase 2 (CMPK2);
interferon α (IFNα)
- From:
Basic & Clinical Medicine
2018;38(7):944-949
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of cytidine/uridine monophosphate kinase 2( CMPK2) in the immune-mediated antitumor effect of IFNα in hepatocellular carcinoma. Methods RT-qPCR and Western blot were used to analyze the expression of CMPK2 in Huh7 after the treatment of IFNα. The CMPK2 overexpressing Huh7 cells were generated by stably infecting with lentivirus. The ATP level in the cells and the supernatant of CMPK2 overexpress-ing Huh7 cells were measured by CellTiter-Glo ATP fluorescence assay. RT-qPCR was applied to test the expression of inflammatory cytokines in macrophages under the treatment of the supernatant of CMPK2 overexpress-ing Huh7 cells. Results The transcription and protein level of CMPK2 were significantly enhanced after the treat-ment of IFNα for 6 hours ( P＜0.01) . CMPK2 increased the ATP level in the cells and supernatant of Huh7 cells ( P＜0.01) . The supernatant of CMPK2 overexpressing Huh7 cells activated the expression of IL1β, IL6 and CCL5 in macrophages( P＜0.01) . Conclusions IFNα increases the expression of CMPK2 in Huh7 cells to activate the expression of inflammatory cytokines in macrophages.
