Knockout of IgG receptor FcγRⅡB aggravates systemic and adipose tissue inflammatory responses in mice
10.3969/j.issn.1001-6325.2018.07.008
- VernacularTitle:敲除IgG受体FcγRⅡB加重小鼠全身及脂肪组织炎性反应
- Author:
Wei ZHANG
1
;
Ting SHU
;
Xiao-Min SONG
;
Bo-Lun LI
;
Jing WANG
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院
- Keywords:
IgG;
FcγRⅡB;
inflammation;
adipose tissue;
lipid metabolism
- From:
Basic & Clinical Medicine
2018;38(7):938-943
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of knockout of IgG receptor( FcγRIIB) on systemic and adipose tis-sue inflammation and fat tissue lipid metabolism treated with high-fat diet ( HFD) . Methods We used 10 male mice with IgG receptor FcγRIIB knockout ( FcγRIIB-/-) and another 10 male FcγRIIB+/+mice as control, and trea-ted them with HFD. At the end of the 17th week, mice were weighed, the blood was taken by cardiac puncture af-ter sacrifice. Adipose tissue was collected to measure inflammation and lipid metabolism. Results Compared with FcγRIIB+/+mice, FcγRIIB-/-mice had significantly increased levels of inflammatory cytokines, IL-6, TNF-α, and IFN-γ in the serum, and increased macrophage infiltration in adipose tissue. M1 polarization gene MCP-1 and TNF-α increased ( P＜0.05) and M2 polarization gene ARG and IL-10 did not differ significantly. However, there was no significant difference in body mass, adipocyte size and expression of lipid metabolism related genes PPARG, CEBPα, FASn, HSL, ATGL, UCP-1 and GLUT4. Conclusions Under HFD treatment, knocking out the IgG re-ceptor FcγRIIB aggravates the inflammatory response of the whole body and adipose tissue, but cannot influence lipid metabolism of adipose tissue and body weight.
