IL-6 and TNF-α promote the differentiation of human BMSCs to TFAs by mimicry inflammatory microenvironment
10.3969/j.issn.1001-6325.2018.07.002
- VernacularTitle:IL-6和TNF-α模拟炎性微环境促进人BMSCs向肿瘤相关成纤维细胞分化
- Author:
Chun-Zhen REN
1
;
Ya-Li LUO
;
Yong-Qi LIU
;
Cheng-Hao LI
;
Yan-Hui ZHANG
;
Feng-Mei WANG
;
Zhi-Wei LU
;
Ling LI
Author Information
1. 甘肃中医药大学 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室
- Keywords:
inflammatory microenvironment;
BMSCs;
TAFs differentiation
- From:
Basic & Clinical Medicine
2018;38(7):901-906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether BMSCs differentiate into TAFs in inflammatory microenvironment simula-ted by IL-6 and TNF-α.Methods The experiment was divided into 9 groups:the BMSCs group,the 50 ng/mL, 100 ng/mL IL-6 intervention group, the 50 ng/mL,100 ng/mL TNF-α intervention group, the 50 ng/mL IL-6+50 ng/mL TNF-α intervention group,the 50 ng/mL IL-6+100 ng/mL TNF-α intervention group,the 100 ng/mL IL-6+50 ng/mL TNF-α intervention group and the 100 ng/mL IL-6+100 ng/mL TNF-α intervention group; After continuous induction for 42 days, BMSCs cell morphology and cycle, TAFs-tagged α-SMA and FAP protein were examined by phase-contrast microscope,flow cytometry and Western blot method. Results Compared with the normal control group, the 100 ng/mL IL-6+50 ng/mL TNF-α intervention group BMSC cell proliferation was significantly promoted;G1phase decreased in proportion and S phase increased;α-SMA and FAP protein expression was signifi-cantly increased (P＜0.05).Conclusions Microenvironment simulated by the 100 ng/mL IL-6+50 ng/mL TNF-α may induce the abnormal change of the BMSCs morphological and proliferative characteristics. TAFs reference mole-cule α-SMA and FAP expression is increased.
