Endothelial progenitor cells with overexpressing angiotensin-converting enzyme 2 reduce monocrotaline-induced pulmonary arterial hypertension in rats
- VernacularTitle:高表达血管紧张素转化酶2的内皮祖细胞降低野百合碱诱导的大鼠肺动脉高压
- Author:
Zhen-Jun LIU
1
;
Jing WEI
;
Meng-Yuan XIAO
;
Li-Qin YU
;
Wen-Yan WEI
;
Zi-Jian PENG
;
Jian-Ying CHEN
Author Information
1. 广东医科大学附属医院 心血管内科
- Keywords:
pulmonary arterial hypertension;
endothelial progenitor cells;
angiotensin converting enzyme 2
- From:
Basic & Clinical Medicine
2018;38(6):751-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of endothelial progenitor cells (EPCs) with overex-pressed angiotensin converting enzyme 2(ACE2) on rat model of monocrotaline(MCT) induced pulmonary arterial hypertension (PAH). Methods EPCs were extracted,purified and identified from the very beginning. The lenti-virus vector carrying ACE2 gene was infected with EPCs in order to prepare the ACE2-EPCs. Besides,the expres-sion of ACE2 and proliferation as well as tube function of EPCs were detected before and after the transduction. The SD rat were randomly divided into control group, MCT induced pulmonary arterial hypertension group (PAH group),ACE2 transduction of endothelial progenitor cells group(lenti-ACE2-EPCs group) and endothelial progenitor cells group (EPCs group).The mean pulmonary artery pressure of rats(mPAP) and right ventricular hypertrophy in-dex (RV/LV+S) were determined in third week after the molding;the lung tissue specimens were stained with HE and the pulmonary arterial wall thickness index(TI) as well as area index(AI) were also calculated;The expression levels of ACE2 in lung tissue were detected by Western blot.Results The mPAP,RV/LV+S,TI and AI detected in PAH group in the third week after the molding were signficantly lower than those in control group(P<0.01); The mPAP,RV/LV+S,TI and AI detected in lenti-ACE2-EPCs group in the third week after the molding were markedly lower than those in PAH group and EPCs group (P<0.01); The expression level of ACE2 in lung tissue of lenti-ACE2-EPCs group was significantly higher than that in PAH group (P<0.01);The mPAP,RV/LV+S,TI and AI detected in EPCs group in the third week after the molding were markedly lower than those in PAH group(P<0.01), while higher than that as compared with control group and lenti-ACE2-EPCs group (P<0.01). Conclusions ACE2 overexpression in endothelial progenitor cells(EPCs) can effectively reduce the mean pulmonary arterial pressure in PAH rat models,and the therapeutic effect of ACE2-EPCs is generally better than that of EPCs.
