Effect of moxifloxacin on pro-inflammatory response in lipopolysaccharide-stimulated mice peritoneal macrophages
- VernacularTitle:莫西沙星对脂多糖诱导小鼠腹腔巨噬细胞炎性反应的影响
- Author:
Na LI
1
;
Ya-Qin LIU
;
Xiu-Bing ZHANG
;
Mei-Xin LI
;
Hong-Xia YUAN
;
Zhen-Yu QIU
Author Information
1. 锦州医科大学第一临床学院临床医学系
- Keywords:
moxifloxacin;
macrophages;
lipopolysaccharide;
toll-like receptor 4;
sphingosine kinase 1
- From:
Basic & Clinical Medicine
2018;38(5):669-673
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of moxifloxacin(MXF)on inflammatory reaction of mice abdominal peritoneal macrophage induced by LPS.Methods The level of TLR4、SPHK1 and NF-κB mRNA was determined by realtime-PCR;The relative levels of TLR4、SPHK1 and NF-κB were determined by Western blot.TNF-αand IL-1 se-creted by macrophages were detected by ELISA.Results At low and medium concentration(8,16 mg/L)of the MXF have inhibitory effect on the increasing of TLR 4,SPHK1,NF-κB expression level and cell supernatant of TNF-αand IL-1 in LPS stimulation of mice peritoneal macrophages.High concentration(64 mg/L)of the MXF promoted the role of inflammation.Conclusions The MXF of low and medium concentrations can inhibit the inflammatory response in LPS stimulation of mice peritoneal macrophages,and high concentration of the MXF showed contrary effects.
