Formaldehyde induced acute inflammation pain leads to enhanced expression of BRD 4 in spinal cord of mice
- VernacularTitle:甲醛致小鼠急性炎性疼痛时脊髓溴结构域结合蛋白4的表达上调
- Author:
You-Ye ZHU
1
;
Hui YANG
;
Jia-Yu ZENG
;
Wen-Yu CAO
;
Zheng-Hai LIU
;
Wei WAN
;
Jie HE
Author Information
1. 南华大学医学院 肿瘤研究所
- Keywords:
bromodomain-containing protein 4(BRD4);
spinal cord;
formaldehyde;
indomethacin
- From:
Basic & Clinical Medicine
2018;38(5):654-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of BRD 4 in the spinal cord and its relationship with acute in-flammation pain induced by formaldehyde in mice.Methods Thirty-six mice were randomly divided into three groups:control group,formaldehyde group and indomethacin+formaldehyde group;25 μL 1%formaldehyde was injected into the right plantar to establish the acute inflammationpain model,while the indomethacin(20 mg/kg) was injected intraperitoneally 1 hour before formaldehyde injection.Then,all the mice were video recored for 1h to observe the spontaneous pain.Then,cell localization of BRD4 in the spinal cord of normal mice was determined by immunofluorescence assasy.The expression of BRD4 in spinal cord was detected by immunohistochemistry and Western blot.Results Immunofluorescence showed that BRD 4 was mainly co-locolized with the neuronal marker NeuN in the spinal cord of normal mice.Formaldehyde injection could induce two-phase spontaneous pain, while indomethacin intervention could only decrease the second phase pain(P<0.05).Furthermore,formaldehyde injec-tion led to significantly enhanced expression of BRD 4 in bilateral spinal cord,which was remarkbly inhibited by in-domethacin(P<0.05).Conclusions Up-regulation of BRD4 in spinal dorsal horn may be involved in the acute in-flammatory pain.
