Impact of celastrol on polarization of mouse peritoneal macrophage
- VernacularTitle:雷公藤红素对小鼠腹腔巨噬细胞极化分型的影响
- Author:
Xiao-Yan LIU
1
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室
- Keywords:
celastrol;
macrophage
- From:
Basic & Clinical Medicine
2018;38(5):643-648
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of celastrol on polarization of mouse peritoneal macrophage.Methods Mouse peritoneal macrophages were isolated by intraperitoneal injection of broth(stimulation method).Cells were divided into control group,celastrol only group, classically activated M1 group and celastrol intervention with M1 group.RT-qPCR and flow cytometry were used to detect the relevant markers(iNOS,Arg-1,CD206,CD11c)ex-pression levels of two polarization states of macrophages(M1 macrophages and M2 macrophages).Results Celastrol could decrease the expressions of revelant markers CD 11c and iNOS of M1 macrophages(P<0.05).Celastrol could enhance the expressions of revelant marker Arg-1 of M2 macrophages(P<0.05).Conclusions Celastrol can sup-press M1 macrophage polarization of mouse peritoneal macrophages.
