Hepatocytes apoptosis induced by β1-adrenoceptor autoantibody is involved in hepatic dysfunction
- VernacularTitle:β1-肾上腺素受体自身抗体通过促进肝细胞凋亡参与小鼠肝损伤
- Author:
Hai-Cun YU
1
;
Yun-Hui DU
;
Shi-Han ZHANG
;
Li YAN
;
Yu-Ming LI
Author Information
1. 首都医科大学基础医学院生理学与病理生理学系
- Keywords:
β1-adrenergic receptor;
autoantibody;
hepatic cells;
apoptosis
- From:
Basic & Clinical Medicine
2018;38(5):626-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of β1-Adrenoceptor autoantibody on liver function.Methods The biologically active of β1-AA was prepared and passive immunization model was established with β1-AA.The bio-chemical parameters of the liver were measured by the automatic serum biochemical analyzer.The liver size, hepatic vein,portal vein velocity were detected by liver ultrasound;hepatocytes apoptosis were tested by tunel stai-ning,annexin V/PI staining and caspase 3 activity detection.Results The biologically active of β1-AA and passive immunization model were established successfully.The ALT and AST of the liver significantly increased and the ALB decreased in the passive immunization process.The apoptosis of the hepatocytes increased,and meto-prolol partially reversed this effect.Conclusions β1-AA may induce hepatocytes apoptosis by β1-adrenergic receptor and participate in the development of liver injury.
