Secreted CTLA-4 fusion Plasmodium falciparum DNA vaccine combined with GM-CSF enhances immune response of mice
- VernacularTitle:分泌型CTLA-4融合恶性疟原虫核酸疫苗联合GM-CSF增强小鼠免疫反应
- Author:
Yu-Hui GAO
1
;
Wei-Wei DENG
;
Chun-Yan WEI
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院病原学系
- Keywords:
malaria;
DNA vaccine;
cytotoxic T-lymphocyte-associated protein-4;
granulocyte-macrophage colony stimulating factor
- From:
Basic & Clinical Medicine
2018;38(5):616-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effects of secreted cytotoxic T-lymphocyte-associated protein-4(CTLA-4)fusion Plasmodium falciparum DNA vaccine combined with granulocyte-macrophage colony stimulating factor(GM-CSF) on humoral and cellular immune responses in mice.Methods The malaria antigen coding sequence fused with CTLA-4 extracellular region of mouse was constructed as eukaryotic secretory expression vector VR 1012-sES312-CTLA,recombinant protein in culture of transfected HEK 293 cells was detected by Western blot.Balb/c mice were co-administrated with VR1012-sES312-CTLA and GM-CSF expression vector.After immunization specific antibody IgG titers and cytokines IFN-γand IL-4 expression levels were evaluated by ELISA and ELISPOT respectively. Results The introduction of CTLA-4 into malaria DNA vaccine system and application of GM-CSF adjuvant signifi-cantly enhanced the specific immune response to the vaccine.Antibody titers in VR1012-sES312-CTLA and GM-CSF co-immunized mice showed a 190-fold increase compared with the simple designed VR1012-ES312 immunization(P<0.001).Conclusions Humoral and cellular immunity induced by malaria DNA vaccine are significantly en -hanced by both dendritic cell-targeting modification and the introduction of GM-CSF molecular adjuvant into the im-mune system.This result provides a new idea for effectively raising the immune response to malaria DNA vaccine.
